Flow Cytometry Resource

You are here

Flow Cytometry Helpline
612-624-7680

Paul Champoux
Program director
champ004@umn.edu
612-625-7165

Therese Martin
Senior Scientist and operations manager
marti229@umn.edu
flowsort@umn.edu
Office: 612-625-3296

The University Flow Cytometry Resource (UFCR) provides comprehensive services and training to the institutional research and educational community.

Flow cytometry uses fluorescent probes to identify and characterize cells or particles. Cells or particles tagged with fluorescent molecules enter the cytometer via a fluid stream. The cells then pass by a series of lasers, each of which emit a specific wavelength of light. The fluorescent probes are excited by the laser and then emit light energy. The fluorescent signal is detected and amplified, then translated into an electronic signal, which is output to a computer workstation. Information about the size and granularity of a cell may be recorded, as well. The result is an electronic file containing the measured information, which may be represented in a graphical format using specific software to interpret the file. This same process may be performed on a cell sorting cytometer which additionally allows for the capture of subsets of the cells or particles to be re-used by the customer in a subsequent procedure.

Exciting changes are happening for UFCR with the opening of the Cancer-Cardio Research Building (CCRB), funding and key internal agreements. Beginning in mid June, the UFCR will be occupying 1200 sq. feet of the new space. We are also making changes to equipment in all of our location.

Overview video