University of Minnesota research uncovers structure, protein elements critical to human function and disease
[Editor's Note - 1/14/15: This paper, from the laboratories of Richard Linck, Ph.D., University of Minnesota, and his collaborator, Daniela Nicastro, Ph.D., Brandeis University, has been recognized by the Journal of Biological Chemistry's Cell Biology Affinity Group as Best Paper of the Year for 2014. Just 21 papers of the thousands published by JBC through the year received the honor. This prestigious honor follows a summer announcement by JBC, naming the paper as Best Paper of the Week.]
New structures discovered within cilia show a relationship between certain proteins and juvenile myoclonic epilepsy. The discovery, made at the University of Minnesota, was named paper of the week in the Journal of Biological Chemistry, and sheds new light on the microstructure of cilia.
Cilia are microscopic, hair-like structures occurring in large numbers on the surface of some of the body’s cells and are involved in movement and perception. Cilia are composed of double microtubules, which are in turn composed of protofilaments.
Richard Linck, Ph.D., professor in the College of Biological Sciences and the Medical School at the University of Minnesota, worked closely with a team to identify specialized ribbons of four protofilaments within the microtubules of cilia. The function of these ribbons had been elusive to researchers, but unlocking the function of these elements improves understanding of how they fit into the bigger picture.
Utilizing cryo-electron tomography and sea urchin sperm tails, researchers identified a single ribbon of four protofilaments in each microtubule, with each microtubule containing a single filament of the protein tektin. Despite the extremely high resolution and protofilament ribbon identifications, there is still a small zone of confusion where the filament meets the ribbon. Thus, it is remains unclear how the filament fits into the structure of the ribbon.
“This discovery may not seem like a giant leap, but it does help us answer some pretty major questions,” said Linck. “This structural mapping, similar to taking apart an engine to understand the subcomponents, allows us to see inside cilia. We’re getting the highest resolution on this structure to date and finding where the parts belong and how they contribute to the overall structure and function.”
As part of the push for structural mapping, Linck and his research team applied biochemical analysis to the ribbon. In this assessment, several proteins have been localized to the ribbon, including tektin and two others casually associated with human disease, including juvenile myoclonic epilepsy.
It is still unclear if the cilia are directly involved in the cause of the epilepsy and other diseases, or if the relevant proteins function in other, non-ciliary ways to cause disease. However, the mapping itself begins to unlock those clues.
Future research is needed to understand the relationship of these ribbon-associated proteins to human disease, as well as to better understand how the filament is structured in the larger ribbon.
Funding for this project was provided by the National Science Foundation award 1024963, the National Institutes of Health grant GM083122, the Characterization Facility, and entities within the University of Minnesota.